brca hereditary cancer mastr plus research application C for the detection of germline variants (snvs, small indels) and cnvs, in 26 (25 genes plus the 3utr of epcam ) disease-associated genes on blood- derived dna . brca hereditary cancer (hc) mastr plus is a disease research panel for the identification of variants in 25 genes and in the 3utr of epcam associated with hereditary breast, ovarian and other related cancers. performance uniformity of amplification (0.2x mean coverage) 96.7 % on target read count 98.6 % dna input as low as 20 ng per plex number of samples/run illumina miseq v3: 93 assay characteristics genes analyzed brca1, brca2, palb2, chek2, bard1, brip1, rad51c, rad51d, tp53, mre11a, rad50, nbn, fam175a, atm, stk11, men1, pten, cdh1, mutyh, blm, xrcc2, mlh1, msh6, pms2 and msh2, and the 3 utr of epcam genomic region analyzed 140.5 kb number of amplicons 561 amplicon length 232-430 bp number of plexes 5 verified with ngs system illumina miseq v3 designed to be compatible with illumina miseq compatible analysis options mastr reporter for brca hc plus application
www.agilent.com not for eu genomics@agilent.com for research use only. not for use in diagnostic procedures. this information is subject to change without notice. pr7000-0721 ? agilent technologies, inc. 2017 published in the usa, september, 2017 5991-8361enuc ordering information cat. no. product name samples mr-0320.024 brca hereditary cancer mastr plus 24 ? mid (molecular identifers) kits are necessary to complete the workflow mastr plus workflow plan run multiplex pcr quality control universal pcr quality control multiplex pcr dilution universal pcr equimolar pooling mixing plexes of single sample purifcation purifcation mixing clean up pooling clean up sequencing data analysis ngs pten cdh1 fam175a men1 pancreas kidney endometrium colon stomach bard1 brca1 brca2 brip1 rad51c rad51d tp53 mre11a rad50 nbn breast ovaries atm palb2 stk11 mutyh chek2 blm xrcc2 epcam pms2 msh2 msh6 mlh1 parathyroid glands disease-associated genes *3 utr of pcam only
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